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Structured Review

Proteintech apaf1
Apaf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+apaf1/IGF2BP3+Antibody/pm41787385-145-38-41
Average 96 stars, based on 212 article reviews
apaf1 - by Bioz Stars, 2026-10
96/100 stars

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Related Articles

Western Blot:

Article Title: APAF1‐Binding Long Noncoding RNA Promotes Tumor Growth and Multidrug Resistance in Gastric Cancer by Blocking Apoptosome Assembly
Article Snippet: .. anti-GAPDH (for WB) Beyotime Cat#AG019 anti-APAF1 (for WB, RIP and LACE) Abcam Cat#ab234436 anti-APAF1 (for IF and IP) Santa Cat#sc-135836 anti-IGF2BP1 (for WB, RIP and IF) Proteintech Cat#22803-1-AP anti-METTL3 (for WB) Proteintech Cat# 15073-1-AP anti-Rabbit IgG (for RIP and LACE) Beyotime Cat#A7016 anti-Mouse IgG (for RIP) Beyotime Cat#A7028 anti-HRP mouse (for WB) Beyotime Cat#A0216 anti-HRP rabbit (for WB) Beyotime Cat#A0208 anti-IHC rabbit (for IHC) Servicebio Cat#G1215 anti-β-actin (for WB) Beyotime Cat#AA128 anti-caspase-9 (for WB) CST Cat#9509 anti-caspase-3 (for WB) CST Cat#9662 anti-cleaved caspase-3 (for IF) CST Cat#9664 anti-Cyt c (for WB and IF) Abcam Cat#ab133504 anti-Ki-67 (for IHC) Servicebio Cat#GB111499 anti-HA (for WB) Beyotime Cat#AF5057 anti-His (for WB) Beyotime Cat#P2233 anti-FLAG (for WB) Sigma -Aldrich Cat#F1804 anti-m6A (for RIP and Dot blot) Abcam Cat#ab208577 anti-GST (for WB) Abmart Cat#MY1901 anti-His (for WB) SMART Cat# SLAB28 Table S8. ..

Immunohistochemistry:

Article Title: APAF1‐Binding Long Noncoding RNA Promotes Tumor Growth and Multidrug Resistance in Gastric Cancer by Blocking Apoptosome Assembly
Article Snippet: .. anti-GAPDH (for WB) Beyotime Cat#AG019 anti-APAF1 (for WB, RIP and LACE) Abcam Cat#ab234436 anti-APAF1 (for IF and IP) Santa Cat#sc-135836 anti-IGF2BP1 (for WB, RIP and IF) Proteintech Cat#22803-1-AP anti-METTL3 (for WB) Proteintech Cat# 15073-1-AP anti-Rabbit IgG (for RIP and LACE) Beyotime Cat#A7016 anti-Mouse IgG (for RIP) Beyotime Cat#A7028 anti-HRP mouse (for WB) Beyotime Cat#A0216 anti-HRP rabbit (for WB) Beyotime Cat#A0208 anti-IHC rabbit (for IHC) Servicebio Cat#G1215 anti-β-actin (for WB) Beyotime Cat#AA128 anti-caspase-9 (for WB) CST Cat#9509 anti-caspase-3 (for WB) CST Cat#9662 anti-cleaved caspase-3 (for IF) CST Cat#9664 anti-Cyt c (for WB and IF) Abcam Cat#ab133504 anti-Ki-67 (for IHC) Servicebio Cat#GB111499 anti-HA (for WB) Beyotime Cat#AF5057 anti-His (for WB) Beyotime Cat#P2233 anti-FLAG (for WB) Sigma -Aldrich Cat#F1804 anti-m6A (for RIP and Dot blot) Abcam Cat#ab208577 anti-GST (for WB) Abmart Cat#MY1901 anti-His (for WB) SMART Cat# SLAB28 Table S8. ..

Dot Blot:

Article Title: APAF1‐Binding Long Noncoding RNA Promotes Tumor Growth and Multidrug Resistance in Gastric Cancer by Blocking Apoptosome Assembly
Article Snippet: .. anti-GAPDH (for WB) Beyotime Cat#AG019 anti-APAF1 (for WB, RIP and LACE) Abcam Cat#ab234436 anti-APAF1 (for IF and IP) Santa Cat#sc-135836 anti-IGF2BP1 (for WB, RIP and IF) Proteintech Cat#22803-1-AP anti-METTL3 (for WB) Proteintech Cat# 15073-1-AP anti-Rabbit IgG (for RIP and LACE) Beyotime Cat#A7016 anti-Mouse IgG (for RIP) Beyotime Cat#A7028 anti-HRP mouse (for WB) Beyotime Cat#A0216 anti-HRP rabbit (for WB) Beyotime Cat#A0208 anti-IHC rabbit (for IHC) Servicebio Cat#G1215 anti-β-actin (for WB) Beyotime Cat#AA128 anti-caspase-9 (for WB) CST Cat#9509 anti-caspase-3 (for WB) CST Cat#9662 anti-cleaved caspase-3 (for IF) CST Cat#9664 anti-Cyt c (for WB and IF) Abcam Cat#ab133504 anti-Ki-67 (for IHC) Servicebio Cat#GB111499 anti-HA (for WB) Beyotime Cat#AF5057 anti-His (for WB) Beyotime Cat#P2233 anti-FLAG (for WB) Sigma -Aldrich Cat#F1804 anti-m6A (for RIP and Dot blot) Abcam Cat#ab208577 anti-GST (for WB) Abmart Cat#MY1901 anti-His (for WB) SMART Cat# SLAB28 Table S8. ..

Incubation:

Article Title: GJB2 c.109G > A mutation activating IFI27-mediated mitochondrial apoptosis pathway leading to hereditary non-syndromic hearing loss.
Article Snippet: Proteins were separated by SDS-PAGE and transferred to NC membranes (Merck, HATF00010). .. Membranes were blocked with 5% BSA (Solarbio, SW3015) for 2 h and then incubated overnight at 4°C with primary antibodies: anti-Caspase 3 (1:1000) (Proteintech, 66470-2-Ig), anti-Caspase 9 (1:2000) (Proteintech, 10380-1-AP), anti-Bax (1:2000) (Proteintech, 50599-2-Ig), anti-Cytochrome c (1:2000) (Proteintech, 10993-1-AP), anti-IFI27 (1:2000) (Abcam, ab171919), anti-APAF1 (1:2000) (Proteintech, 29022-1-AP), and anti-β-actin (1:5000) (Proteintech, 66009-1-Ig) as internal reference. .. Membranes were then incubated with secondary antibodies (1:10000) (Proteintech, SA00001-1, SA00001-2) at 37°C for 2 h, washed three times, and visualized using an ECL chemiluminescence imaging system (Savior, SCG-W2000).

Article Title: The Yeast and Hypha Phases of Candida krusei Induce the Apoptosis of Bovine Mammary Epithelial Cells via Distinct Signaling Pathways.
Article Snippet: Subsequently, the separated proteins were deposited onto polyvinylidene fluoride (PVDF) membranes obtained from Epizyme Biotech in Shanghai, China. .. Following a 2 h period of blocking in a solution of TBS containing 0.05% Tween 20 and 5% non-fat milk, the membranes were subsequently subjected to overnight incubation at 4 ◦C with antibodies specific to the proteins of interest: primary rabbit antibodies against β-actin (1:1000), anti-total ERK1/2 (1:1000), anti-total JNK (1:1000), anti- phospho-ERK1/2 (1:1000), anti-phosphor-JNK (1:1000), anti-cleaved-caspase-3 (1:1000), anti-cleaved-caspase8 (1:1000), anti-cleaved-caspase-9 (1:1000), anti-caspase-3 (1:1000), anti-caspase-8 (1:1000), anti-Bim (1:1000), anti-TLR2 (1:1000), anti-TLR4 (1:1000), anti- MYD88 (1:1000), anti-FOXO4 (1:1000), anti-phospho-FOXO4 (1:1000), anti-APAF1 (1:1000), anti-Fas ligand (1:1000) (all from Bioss, Beijing, China); anti-caspase-9 (1:1000) and anti-Bax (1:1000) (from Proteintech, Wuhan, China); anti-total P38 (1:1000) (from Abcam, UK);anti-phospho-P38 (1:1000) (from ThermoFisher, Waltham, MA, USA); and anti-Bcl-2 (1:1000) (Santa Cruz Biotech, Santa Cruz, CA, USA). .. In order to perform suitable secondary antibody incubations, we utilized horse radish peroxidase (HRP)-conjugated goat anti-mouse or goat anti-rabbit antibodies (Proteintech, Wuhan, China) at a dilution of 1:5000.

Article Title: GJB2 c.109G > A mutation activating IFI27-mediated mitochondrial apoptosis pathway leading to hereditary non-syndromic hearing loss
Article Snippet: Proteins were separated by SDS-PAGE and transferred to NC membranes (Merck, HATF00010). .. Membranes were blocked with 5% BSA (Solarbio, SW3015) for 2 h and then incubated overnight at 4 °C with primary antibodies: anti-Caspase 3 (1:1000) (Proteintech, 66470-2-Ig), anti-Caspase 9 (1:2000) (Proteintech, 10380-1-AP), anti-Bax (1:2000) (Proteintech, 50599-2-Ig), anti-Cytochrome c (1:2000) (Proteintech, 10993-1-AP), anti-IFI27 (1:2000) (Abcam, ab171919), anti-APAF1 (1:2000) (Proteintech, 29022-1-AP), and anti-β-actin (1:5000) (Proteintech, 66009-1-Ig) as internal reference. .. Membranes were then incubated with secondary antibodies (1:10000) (Proteintech, SA00001-1, SA00001-2) at 37 °C for 2 h, washed three times, and visualized using an ECL chemiluminescence imaging system (Savior, SCG-W2000).

Article Title: Propofol Ameliorates H9c2 Cells Apoptosis Induced by Oxygen Glucose Deprivation and Reperfusion Injury via Inhibiting High Levels of Mitochondrial Fusion and Fission
Article Snippet: Cell lysates were separated on 6, 10, 12% SDS-PAGE, and transferred to polyvinylidene difluoride membranes (PVDF, Millipore, Billerica, MA, United States). .. The membranes were blocked and incubated with primary antibodies against anti-Drp1 (1:1000, BD Biosciences, United States), anti-pDrp1 (1:1000, CST, United States), anti-Fis1 (1:1000, Proteintech, China), anti-Mfn1 (1:1000, Proteintech, China), anti-Mfn2 (1:1000, CST, United States), anti-Opa1 (1:1000, BD Biosciences, United States), anti-ERK (1:1000, CST, United States), anti-pERK (1:1000, CST, United States), anti-Cytochrome c (1:1000, CST, United States), anti-Bax (1:1000, CST, United States), anti-Bak (1:1000, CST, United States), anti-Apaf1 (1:1000, Proteintech, China), anti-Caspase 9 (1:1000, CST, United States), anti-Caspase 6 (1:1000, CST, United States), anti-Caspase 7 (1:1000, CST, United States), anti-Caspase3 (1:1000, CST, United States), anti-COX IV (1:1000, CST, United States) and anti-GAPDH (1:1000, CST, United States) at 4°C overnight. .. After primary antibody incubation, the membranes were incubated with either goat anti-mouse or goat anti-rabbit horseradish peroxidase-conjugated secondary antibodies, at room temperature for 2 h. Immunoreactive bands were developed with an electrochemiluminescence (ECL) reagent (Thermo-Pierce, Rockford, IL, United States).

Blocking Assay:

Article Title: The Yeast and Hypha Phases of Candida krusei Induce the Apoptosis of Bovine Mammary Epithelial Cells via Distinct Signaling Pathways.
Article Snippet: Subsequently, the separated proteins were deposited onto polyvinylidene fluoride (PVDF) membranes obtained from Epizyme Biotech in Shanghai, China. .. Following a 2 h period of blocking in a solution of TBS containing 0.05% Tween 20 and 5% non-fat milk, the membranes were subsequently subjected to overnight incubation at 4 ◦C with antibodies specific to the proteins of interest: primary rabbit antibodies against β-actin (1:1000), anti-total ERK1/2 (1:1000), anti-total JNK (1:1000), anti- phospho-ERK1/2 (1:1000), anti-phosphor-JNK (1:1000), anti-cleaved-caspase-3 (1:1000), anti-cleaved-caspase8 (1:1000), anti-cleaved-caspase-9 (1:1000), anti-caspase-3 (1:1000), anti-caspase-8 (1:1000), anti-Bim (1:1000), anti-TLR2 (1:1000), anti-TLR4 (1:1000), anti- MYD88 (1:1000), anti-FOXO4 (1:1000), anti-phospho-FOXO4 (1:1000), anti-APAF1 (1:1000), anti-Fas ligand (1:1000) (all from Bioss, Beijing, China); anti-caspase-9 (1:1000) and anti-Bax (1:1000) (from Proteintech, Wuhan, China); anti-total P38 (1:1000) (from Abcam, UK);anti-phospho-P38 (1:1000) (from ThermoFisher, Waltham, MA, USA); and anti-Bcl-2 (1:1000) (Santa Cruz Biotech, Santa Cruz, CA, USA). .. In order to perform suitable secondary antibody incubations, we utilized horse radish peroxidase (HRP)-conjugated goat anti-mouse or goat anti-rabbit antibodies (Proteintech, Wuhan, China) at a dilution of 1:5000.



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